Isolation Toxoplasma gondii from aborted fetuses and Sheep then study of the prevalence in aborted women in Karbala governorate

Abstract

The aim of this study is to isolation Toxoplasma gondii from sheep and its fetus’s. Then the study conducted to determine the distribution of the parasite in women who have experienced abortion and threats in the province of Karbala and the surrounding areas. The zoonotic aspect investigated the different sources for infection with toxoplasmosis ,generally our results proved that ovine meats is an important sources for transmission of toxoplasmosis; the percentage of positive result with tachyzoites and tissue cysts was 80 % after pepsin digestion .When the comparative study of the different organs of aborted fetus, we found that the brain was the more sensitive organ and considered predilection site for Toxoplasmal infection, other organs such as liver, kidney, lung, heart , muscles showing less tendency for this infection. The severity of infection with Toxoplasma was classified Toxoplasma infection in aborted fetus to sever, moderate and mild infection according to the number of tissue cysts in brain. The cases of severe infection were predominated when compared with mild and moderate infection. The immunological aspect of this study showed the presence of immunoglobulin G in the placenta 22 % on using modified latex agglutination test with 2-ME and 3 % on using indirect ELISA. The prevalence of the parasite Toxoplasma condi in women who have undergone abortions and threatened abortion in the city of Karbala, whose ages ranged between 25-34 years. Direct agglutination method and Elisa technique have been diagnosed serologically for 90 blood samples, the result showed that the infection rate was 18.88% by agglutination test and 13.33 by Elisa test. Infection rate was 10.71%,33.,21.05% , 33.33% for threatened abortion , first time , t% for threatened abortion ,one time and two times aborted women respectively by Elisa technique. Rate of measured IgM was 1.23, 1.55 and 1.44 for one time, two times and three times aborted women respectively.