Assessment of TLR2 & TLR4 in urine of Iraqibacter isolated from urinary Tract infection of Baghdad hospitals

Abstract

Acinetobacter baumannii has grown to be a major source of worry. It has gained particular notoriety in the recent desert conflicts in Iraq, earning the nickname "Iraqibacter." Although most A. baumannii infections have been studied in the context of pneumonia and bloodstream infections. Urinary tract infections (UTIs) are occasionally caused by A. baumannii . Three hundred and forty-six urine samples were collected from several Baghdad hospitals in Karkh. Samples were taken from different ages and genders. This study has been done during the period from the first of November 2021 to the end of March 2022. Among all 3466 urine samples with positive culture, only 14 cases revealed Acinetobacter baumannii isolates (about 4%) , the isolates of positive cultured urine were identification by conventional methods for identifying Acinetobacter baumanniii (i.e. Iraqibacter). The non-lactose and partially lactose fermenting colonies on MacConkey agar and non-hemolytic vague creamy colonies on blood agar, and   cultured on CHROM agar selective medium for A.baumanniii, and  the isolates were definitely identified by using the VITEK- Toll like receptors 2(TLR2) and Toll like resptors 4 ( TLR4) concentration in urine was estimated by specific commercial sandwich enzyme-linked immune sorbent assay ELISA kit . The present results demonstrated that the TLR2 level elevated in urine of patients in comparsion to control with a high statistical significance The current results also showed that TLR4 concentration in urine of patients was  slightly elevated with non-significant difference .