Effect of Alcoholic Extract from Three Plants that is Rich of Flavonoids on two Virulence Factors in E. coli

Abstract

In this study, the flavonoid content was estimated by HPLC for Vitis vinifera, zingiber officinale and date palm phoenix dactylifera after alcoholic extracts (Ethanolic extracts) and exposure of Escherichia coli that were isolated in 30 Covid-19 patients.  From the medical isolation unit in Salah al-Din Hospital, Tikrit city, Republic of Iraq, bacteria were exposed to plant extracts containing flavonoids at different concentrations and the minimum inhibitory concentrations (MIC) were estimated. Inhibition zones were observed at all concentrations, and then the polymerase chain reaction (PCR) was performed.  On two of the most important genes that encode two of the virulence factors in E.coli bacteria, the fimA gene and the PgaD gene, and this procedure showed clear effects on the level of the mentioned genes through PCR technology, which opens the door to the possibility of wider utilization and the development of effective compounds  And its exploitation from various plants with a medical reputation known historically "in light of the health difficulties we are going through due to a pandemic  Corona virus.