The Detection of Il-6 -174 G/C Gene and It’s Associated with Cervical Cancer Among Iraqi Women

Abstract

Our study included thirty of cervical cancer samples for Iraqi women, their ages ranged from 27 to 78 year, and twenty-five of the controls (outwardly healthy), their ages ranged from 13 to 50 year. We confined the frequency of IL-6 Gene polymorphism by using Tetra-amplification refractory mutation system-polymerase chain reaction technique (TARMS PCR). Also, we determined the association of IL-6 Gene polymorphism in position (-174 G/C) with cervical cancer for Iraqi women. Data showed significant difference in genotypes frequency of IL-6 Gene polymorphism with cervical cancer and control.

The genotype of GG homozygotes show high frequency in cervical cancer patients was ratio 63.33%, comparison with control was 28% and showed associated with etiological fraction of cervical cancer risk, ratio was 49.1%, and OR 4.44, CI 95% was 1.44 to 13,68. The genotypes of GC Heterozygotes and CC homozygotes show high frequency in control ratio was 24% and 48% respectively, and OR was 0.49 and 0.33 respectively, CI 95% was 0.12 to 1.92 and 0.11 to 1.02 respectively. and present with preventive fraction of cervical cancer risk. The G allele show high frequency in cervical cancer, comparison with control ratio was 70% and 40% respectively, and present related with etiological fraction for cervical cancer, while C allele showed associated with preventive fraction of cervical cancer risk. Our findings demonstrate that the GG genotype of IL-6 gene in position -174 G/C may represent of cervical cancer risk development of cervical cancer patients in