Antibiotic Resistance and Biofilm Composition of E. coli, K.Pneumoniae, S.Aureus Isolated from Surgical Procedures

Abstract

Collected (154) samples of patients with surgical inflammation reviewed and lying at Ramadi General Teaching Hospital and Women's and Children's Hospital in Ramadi (Anbar province), the process of collecting samples began from 4/10/2021 to 1/2/2022 i.e. (October 4, 2020) 21 to February 2022, the study included patients (caesarean sections, appendicitis, tonsils, colon-hemorrhoids), both sexes and of different ages, to investigate the presence of E.coli, K.pneumoniae, S.aureus bacteria in the membranes of surgeries. The results indicated that E.coli bacteria are the most isolated bacterial species in the current study, if they rank first among bacterial species that take the digestive system as a natural habitat, the resulting yeast may be the cause of internal source contamination such as surgery for the oligmony or appendix, or be external acquired as hospitals, and appeared with K.pneumoniae bacteria isolated from patients with inflammation of surgical operations by (12.34%), It is an opportunistic bacteria because it has many fierce factors that enable it to resist the antibacterial serum, since the natural location of its presence is the upper section of the respiratory system, and as it appeared bacteria S.aureus by (9.74%), if it is one of the most important opportunistic bacterial races and its presence in the surgical site does not constitute any shock as it is responsible for most cases of injuries and ulcers. In this study, 59 isolations were tested, divided between (25) E. coli (19) ciliary isolation, while Staphylococcus aureus was (15) isolation, to determine the susceptibility of bacteria to the production of the membrane by the method of microcalculation dishes, the results showed 30(50.84%) isolation created membrane Strong biosphere, 13 (22.03%) of insulation produced an average biosphere, 14 (23.72%) isolation produced a weak life membrane, the study showed 100% bacteria sensitivity to anti-Ceftriaxone for bacterial species E.coli, K.pneumoniae, S.aureus, And 100% Cefazolin for E.coli, K.pneumoniae.