Isolation of Bifidobacterium Ssp from Human Breast milk and Study Their Susceptibility to Antibiotics

Abstract

Background: Breast milk has beneficial impacts on human health and is a significant source of nutrition for newborns. One intention is that the micro biota in this human milk contains advantage bacteria.  Purpose: This study was undertaken to isolate of Bifidobacterium from breast milk and study the susceptibility of Bifidobacterium ssp isolate to antibiotics.  Methods: The sample of breast milk have been collected from breastfeeding women at Al-Zahra Teaching Hospital and Al-Kut Hospital (from the fourth day to two months after birth),90 samples of human breast milk were subjected to this study .3 ml of milk sample should be placed under aseptic conditions in a sterile tube to promote anaerobic growth.  Results: The colonies of Gram –positive, catalase –negative bacteria from particular medium of typical bifidobacterial organisms were identifided at the genus level after incubation. Using specific primer and 16 rRNA gene sequencing, for the confirmed Bifidobacterium ssp. populations in breast milk. 60 out of 90 of collected breast milk samples. Bifidobacterium ssp. isolated were resistant to vancomycin streptomycin and nalidixic acid, and intermediate resistant to neomycin, tetracycline, but susceptible to chloramphenicol and gentamycin.