Molecular Detection of PVL Genes in Methicillin-Resistance Staphylococcus Aureus that Isolated from Ramadi Hospitals Patients

Abstract

Pantone Valentin Leucocidin (PVL) is a cytotoxin produced by methicillin-resistant Staphylococcus aureus, and it is one of the most dangerous pathogens that threaten health, as it causes infections of the skin and soft tissues. The polymerase chain reaction (PCR) is applied to detect MRS and determine the prevalence of virulence genes, the most important of which are genes luk-pv (lukS-pv,lukF-pv), The species is detected using the 16SrRNA gene. 125 clinical samples were collected from different cases (wounds, burns, urine, suppurations and abscesses of the skin, ear) from the main hospitals in Ramadi (Al Ramadi Teaching, Women and Children Hospital) for the period from month (11-2021 to 1-2022). Twenty-six MRSA isolates were identified by PCR by 16SrRNA gene. The isolates were 96% luk-pv positive. The results showed a diverse profile of resistance to 9 antibiotics, as the total resistance was 100 %against clindamycin, cefoxtin, and erythromycin. It also gave a high resistance to azithromycin by 96.15%. While the resistance was moderate towards lincomycin and tetracycline (42.30%, 46.15%) respectively, whereas the resistance to ceftriaxone, gentamycin and doxycycline was low (11.53%, 11.53%, 3.84%) respectively. While the sensitivity of the isolates was high against gentamycin by 80.76% and moderate against doxycycline by 65.38%. Monitoring and research on MRSA carrying the PVL gene should continue to provide important insight into the spread of these resistant pathogens.