Study Effect of Different Low-Level Laser Intensity on Absorbances and Fluorescence Properties of Blood Components
Keywords:
Laser radiation, Human blood radiation, Fluorescent, and absorption of proteins.Abstract
Low-level laser intensity (LLLT) had been employed to induce and diagnose several biological activities within tissues and blood. The project study was designed to explain the effects of LLLT on absorbance and fluorescence features of different blood components including (plasma, serum, serum without proteins, and precipitated serum proteins. LLLT (437nm, 532nm, 632nm) was used to irradiate of blood drive component for 10 minutes with power (20mw, 28mw, 40mw). Results of non-radiated components showed there was a high peak (absorbance) of precipitated protein flowed by serum, plasma, and serum without proteins. The irradiated plasma samples showed increased absorbance with blue laser in comparison with other components whereas irradiated serum samples indicated high absorbance with red laser compared to other components precogitated serum protein recorded increase opted density with green lases with lower than control. Samples of serum without proteins pointed out a significant increase of absorption in green and blue laser compared to control and other laser radiation. Regarding fluorescent properties of blood components, plasma samples showed a higher peak with red laser compared to other laser radiated samples. On the other hand, serum radiated samples confirmed a remarkable peak with green laser compared to other samples. However, precipitated radiated serum protein was a significantly lower fluorescence peak when matched with those control samples. Also, samples serum without proteins and irradiated with different laser wavelengths showed lowering fluorescence peaks in matching with control (non-irradiated samples). In conclusion, the results which are mentioned above can be attributed to different densities of components of blood because isolation of proteins (clotting and serum proteins), as well as the photo-biochemical modulation or changes induced by laser radiation, can affect absorption and fluorescence peaks of that component.
