Simultaneous Estimation of Trifluoparazine by UV-VIS Spectroscopy using of the Oxidative Coupling Reaction with Reagent Naphthalene-1, 5-Diamine

Abstract

A simple, rapid and sensitive spectrophotometric method was described for the determination of Trifluoparazine the method is based on the oxidative coupling reaction between Trifluoparazine and Naphthalene-1,5diamine in the presence of Potassium Iodate to form violet colored product with maximum absorptions at 535nm, which is soluble in water. Beer's law is obeyed in the concentration range of 10 to 50㎍.ml-1 with a molar absorptivity of 0.7975×104 L.mol-1.cm-1, and Sandell's index of 0. 0602㎍.cm 2, respectively. The correlation coefficient of 0.9994, with recovery average % of 99.6 Limit of detection (LOD), limit of quantification (LOQ) of 0.782 ㎍.ml- 1and 2. 609㎍.ml -1 with relative Error (RE) % of -0.2 to -0.5 and a relative standard deviation (RSD) % of 0.561 to 0.231. The proposed method has been used to successfully determine Trifluoparazine in pharmaceutical formulations (Tablet).