Probiotic Inhibit Biofilm Formation for Acinetobacter Baumannii

Abstract

Acinetobacter baumannii bacteria have been classified as the most problematic in hospitals, and they need to be researched and developed significantly new antibiotics by the World Health Organization (WHO), and as an urgent public health threat by the Centers for Disease Control and Prevention (CDC). In this study, twenty-five isolates (20.83%) of these bacteria were isolated from a total of 120 samples collected from various clinical sources (burns, blood, wounds, sputum) from patients with various medical injuries from Baquba Teaching Hospital in Diyala Province for the period from (November 1, 2021 to February 1, 2022). According to the physical and microscopic diagnosis and the use of VITEK2 and molecular detection, all diagnosed isolates are A. baumannii bacteria. All MDR insulation showed complete resistance to the Colistin antibiotic. The results of the appearance detection of some factors of virulence showed that most isolates have the ability to adhere to epithelial cells and the formation of the biofilm and the formation of the biofilm is associated with resistance to antibiotic isolates (MDR) and on this basis studied (10) clinical isolates and their composition ability of the biofilm and to different degrees were seven very strong isolates and by (70%), 1 (10%) medium formation and two weakly formed isolates of the biofilm so took the ratio (20%). The susceptibility of isolates to the formation of gelatinase was high by (67%), which mean that (19) isolates was formed of gelatinase, while the enzyme lipase was 22) isolated and 88% produced by this enzyme, while the appearance detection of the capsule was the result 1 (4%). The results of the molecular detection of the gene (Bab) showed the presence of one isolate carrying this gene and a ratio of (10%). RT-PCR technology was used to measure the gene expression of the gene studied (Bab) by extracting DNA (RNA) for A.baumannii bacteria before and after treatment with probiotic, one bacterial isolates of A.baumannii was treated with two types of probiotic Lactobacillus plantarum, Lactobacillus acidophilus and note how much they affect gene expression when MIC was used (for bacteriophage CFS at a concentration of 12.5 µg/mL) the results showed an effect of Lactobacillus plantarum bacteria on the gene expression of A. baumannii causing full inhibition of Bap gene when treated LP.