Immunological Effects Caused by Klebsiella pneumoniae Infections in White Rats and Select of Inhibitory for Rosmarinus Officinalis Extract’s

Abstract

This study was conducted in the laboratories of the Department of Biology, College of Education for Pure Sciences, Tikrit University, and the study aimed to isolate and diagnose Klebsiella pneumoniae,and study its immunopathology after infection in white male mice, 65 clinical samples were collected from sputum. Twelve isolates belonging to Klebsiella pneumoniae were diagnosed, and they constituted 35.2% of the total 34 gram-negative isolates. 25 laboratory animals were used, distributed into 5 groups, 5 animals per group. The first group represented the control group, while the second group was injected with a bacterial suspension in a volume of 1.5 ml and was not treated with any substance, while the third group was injected with a bacterial suspension with a volume of 1.5 ml and was treated with aqueous extract of rosemary leaves, and the fourth group was injected with a bacterial suspension at a volume of 1.5 ml and was treated with alcoholic extract rosemary leaves, As for the fifth group, it was injected with the bacterial suspension in a volume of 1.5 ml and was treated with the antibiotic Imipenem. The results of the study showed a decrease in the rate of IL-1α, which amounted to 59,714 pg / ml compared to the control group, and an increase in the rate of IL-17 was recorded, which amounted to 469.153 pg / ml The results confirmed the presence of a cellular and humoral immune response caused by infection with the bacteria Klebsiella pneumoniae.