Bassociation among Biofilm Formation and Antibiotic Resistance Genes in Klebsiella Pneumoniae Isolates from Urinary Tract Infections

Abstract

To conduct this investigation, researcher collected 140 samples from individuals with UTIs. The collecting period from October 2021 through March 2022. The results revealed that 50 (35.7%) specimens gave a positive culture (Klebsiella pneumoniae), and the other 90 (64.3%) specimens were considered negative culture (other causative agent). There were 17 isolates of K. pneumoniae able to develop a biofilm as strong, 24 as moderate, and 9 as weak, and on tissue culture plates the number of biofilm-forming isolates was 45 (90 %) and the non or weak biofilm-producing isolates were 5 (10 % ). To study the sensitivity of those isolates against general antibiotics, 9 types of different antibiotics were tested using Kirby-Bauer disc diffusion method. The findings, which depict the isolates' antimicrobial profiles, show that the isolates' susceptibilities to antibiotics differed. However, ciprofloxacin was the highly effective antibiotic against for all tested isolates, while, amoxicillin was the lesser effective antibiotic against for all tested isolates. Determination of the minimum inhibitory concentrations (MIC) anti biogram testing was performed with the automated VITEK-2 compact system. Bacterial isolates' sensitivity to antibiotics differed according on their anti biogram profile, as seen in the findings. Biofilm formation in different test Klebsiella was reduced by using some antibiotics such as tetracycline, naldixic acid, cefepime, amoxicillin, ciprofloxacin, tobramycin, gentamycin, and amikacin, this antibiotics called anti biofilm formation.