Inhibitory Effect of Hesperidin on Kidney Type L-Glutaminase

Abstract

Citrus limon was extracted with 80% methanol, Then HPLC analysis for Citrus limon methanolic extract represent on hesperidin peak at retention time 14.80 ppm. Invitro inhibition assay for standard KGA with purified hesperidin gave an inhibition of 65.33 % compared to CB-839 positive conttrol. Finally, the cytotoxic effect of purified hesperidin was assessed against breast cancer cell line (AMJ13) and normal cell line (HBL-100) via MTT assay. Demonstrated the strongest and significantly higher cytotoxic activity against AMJ13 especially at 500 µg/mL of 80.67±1.45%, with IC50 value was 22.45µg/ml.while compound showed a variation in toxicity on normal cell line HBL-100.