Detection of Relationships of Immunohistochemical, Molecular and Electron Microscopy Study of EGFR of Mutation in NSCLC in Patients

Abstract

Background: Mutations in the gene EGFR can lead to expression of mutant EGFR proteins that accumulate in tumor tissues and is responsible for approximately 1.76 million deaths each year worldwide. The aim of this study by found relationships of EGFR mutation status NSCLC between mutation-specific immunohistochemistry (IHC), molecular biology and electron microscopy. Methods: 30 tissue blocks embedded in paraffin wax of NSCLC patients were evaluated for (11 females and 19 males) while controls were 20 samples from healthy, detection of protein expression in by EGFR IHC and used Molecular study, electron microscopy. Results: Our study results have find the mean age of patients group was 51.84±17.70 years was don study shows no correlation between age of patients with the stage,site and grade of tumor, while recorded in immunohistochemical method highly significant difference between EGFR expression with age, gender, grade and stage tumor, we found not correlation between the immunohistochemical of EGFR protein and genetic study in NSCLC but its correlation with electron microscopy in (P < 0.05). Conclusion: Our results indicate the presence of relationships clear between Clinicopathologic characteristic in patients of immunohistochemistry EGFR and Electron Macroscopically but were found not clear role with Molecular study of NSCLC patients.