Green Synthesis of silver nanoparticles from Atriplex nummularia leaves extracted as antifungal against Candida albicans.

Abstract

Nanotechnology is one of the driving reasons for the upcoming knowledge revolution, particularly in the biomedical sectors and the development of antimicrobial alternatives This study included using the well diffusion method and the detection of the minimum inhibitory concentration to estimate the antifungal activity of green silver nanoparticles (AgNPs) synthesized by the green chemistry process from the leaf extract of Atriplex nummularia and evaluated their antimicrobial activities for three pathogenic isolates of Candida albicans through Minimum Inhibitory concentration (MIC). Atriplex nummularia leaves extract and silver nitrate were used to synthesize AgNPs; the aqueous plant extract acts as a reducing and stabilizing agent for silver nanoparticles.Fourier transforms infrared spectroscopy (FTIR),Field emission-scanning electron microscope (FESEM),X-ray diffraction (XRD), and Atomic force microscope(AFM) were used to evaluate the produced nanostructure. X-ray diffraction analysis was used to determine the AgNPs' crystalline nature. FTIR analysis appeared bundle the characteristic of the hydrogen-bonded OH group, which may be due to the formation of nanoparticles in the aqueous phase, FESEM appeared spherical in shape and around (17.50-127.87) nm in size,AFM ranged between (1-42.36) nm,XRD appear the average crystalline size of AgNPs was found between( 6-26 )nm silver nanoparticles appeared an antifungal activity at concentration of (512, 256, 128, 64) μg/mL respectively, while showing no inhibition and antifungal effects on study isolates at the concentration of( 32,16,8,4,2)μg/ml. The antifungal activity of the produced silver nanoparticles from Atriplex nummularia leaves extract was proven that its simple, inexpensive, safe, and effective alternative to standard antifungal medicines