Synergistic Activities of Tetracycline with CuO Nanoparticles Study of its Activity on Antibiotic-Resistant Bacteria

Abstract

The synergy between antibiotics and nanoparticles is one of the most significant ways for combating antibiotic resistance. Tetracycline/copper oxide nanoparticles were produced utilizing a chemical precipitation approach and a physical process including ultrasound in this research. Tetracycline nanoparticles were mixed with CuO nanoparticles. The findings demonstrated a consistent distribution of copper oxide nanoparticles in the tetracycline particle matrix. The resulting nanocomposites were characterized using XRD, EDX, and SEM techniques (CuO) and matched with the (ICDD) database of the International Center for X-ray Diffraction [ICDD card no. 41-0254] The average crystal size was 44.83 nm. The average crystal size was 22.37 nm. The TCS with CuO synergy was studied. By assessing the lowest inhibitory concentration of two types of bacteria using ELISA equipment, the antibacterial compatibility and synergistic studies of tetracycline/ CuO nanoparticles were compared to normal tetracycline particles acquired from Samarra Pharmaceutical Laboratory. The MIC of synergistic TCs with CuO NPs against Staphylococcus was 32 μg/ml, indicating high efficacy in killing germs. In comparison, the MIC of standard TCs was 512 μg/ ml, indicating an increase in efficacy of the above compound compared to standard TCs. While the synergistic TCs with CuO NPs did not change the MIC concentration towards pseudomonas aeruginosa compared to the standard TCs. Hemolysis was also used to investigate the toxicity of the compound (TCs + CuO NPs). The results of the tests revealed that the chemicals were safe. It is considered non-toxic at all doses of TCs + CuO NPs because it did not break up the platelet and had no harmful effect.