Effectiveness of Phytochemicals extracted from Mirabilis jalapa (L) Roots on Some Antibiotic resistance Bacteria

Abstract

This study was conducted in the laboratories of the College of Science / University of Kufa from October 2021 to March 2022, the roots of Mirabilis jalapa(L.) plant was extracted by Soxhlet and Vortex methods using methanol solvent 96% with three temperatures (30,40 and 50 C°) degrees to obtain the best alcoholic extract. GC-MS analysis was conducted to identify the quality and quantity of phytochemical compounds in crude extracts used in this study.

The extracts were chosen from the followed methods depending on the amount of active compounds in each extract diagnosed by GC-MS,. Two types of extracts (Soxhlet +40C° and Vortex +50C°) were chosen as separate treatments in the subsequent experiments.

Bacteria resistant to antibiotics Staphylococcus aureus (48.57%) and Pseudomonas aeruginosa (37.14%).were obtained from the post Graduate Microbiology Laboratory / College of Science / University of Kufa.Bacterial samples were diagnosed by morphological, microscopic and biochemical tests. The diagnosis was confirmed using the Phytek device,

Three concentrations of roots extract 1, 5 and 10 mg/ml were prepared for each of the selected extraction methods and Temperature degree to evaluate the antibacterial activity at three replicates for each concentration using the well diffusion method.

The qualitative analysis was studied for the detection phytochemicals using GC-MS analysis was conducted to identify the quantity of phytochemical compounds in crude extraction methods used in this study. The higher phytochemicals reported 46 peaks indicating in roots of M.jalapa plant extract used Soxlate apparatus at 40°C by GC-MS chromatography. The results showed that all extract concentrations gave bacterial growth inhibition and all species were more affected by increasing these concentration study Also, the results in current study reported that the highest growth inhibition rate of Pseudomonas aeruginosa was for the methanol extract by soxlat methods at 40C° with an inhibition rate (23.33mm), while the lowest inhibition growth rate was for the alcoholic extract using Vortex methods at 30 C° an inhibition rate (12.41mm) for the Staphylococcus aureus bacteria. Also, the grater bacteria affected by crude plant extract was Pseudomonas aeruginosa at 10 mg/ml.