Molecular Basis of Programmed Cell Death-1 Gene and its Association with Encoding Protein Levels of Iraqi Women with Polycystic Ovarian Syndrome
Keywords:
Programmed cell death protein-1, Polycystic ovary syndrome, Single-nucleotide Polymorphism. Gene. PDCD-1.Abstract
Background: Tumors, immunological disorders, and inflammation are all significantly reduced by programmed cell death-1 (PD-1) Polycystic ovarian syndrome (PCOS) patients have a uniform clinical feature of chronic inflammation. PD-1 ligand can produce T-cells apoptosis and produce immunosuppressive effects. The level of PD-1 was increased in both CD4+ and CD8+ T cells from infertile patients with PCOS.
Materials and Methods: Whole blood samples of 80 PCOS patients and 80 healthy people were collected from Dec., 2021 to June, 2022 at the gynecological and obstetric teaching hospital, Kerbala health directorate, Iraq with age range between (18 -40) years. The Rotterdam criteria-2003 was accepted to PCOS females. BMI and WHR were determined. DNA was extracted and stored at -20 C until use. Primers designed based on NCBI database. Genotypes detected using the Taq Man allelic discrimination real-time PCR. Hormonal status was determined by cobas e411. Elabscience /USA ELISA kit was used to determine PDCD-1 level in serum.
Results: The PCOS women had a significantly higher frequency of PD-1(rs2227982) GA genotype. P-value (0.0151), OR (2.728) and an allele had significance P-value (0.0237), OR (2.393). A significant elevation of PDCD-1 level (P=0.01) were obtained in PCOS patients group and there is significant association between PD-1 level and PD-1 gene (rs2227982) GA genotype, P value = 0.0002.
Conclusion: The observed data indicate that the PDCD-1 protein significantly increase in PCOS patients. PDCD-1(rs2227982) genotype GA is associated with a susceptibility to PCOS and its pathogenesis. It also demonstrated that PD-1 rs2227982 was significantly associated with GA genotype in PCOS Iraqi patient.
