Association Between Covid-19 Risk and The Genotypes (Gg, Gc and Cc) And Its G and C Alleles for Interlukin-6 (Il-6) Gene Polymorphisms

Abstract

COVID-19 disease is a highly contagious respiratory inflammation occur by viral infection (SARS-CoV-2). Cytokines are proteins that mediators of dysregulated immunity, and stimulated of immune response such as interlukin-6 (IL-6). Our study included two hundred and twenty samples of COVID 19 patients of Iraqi population, their ages ranged from 21 to 79 year (mean of ages 37.8 ± 1.4) and one hundred and twenty of the controls (outwardly healthy), their ages ranged from 19 to 54 year (mean of ages 27.9 ± 1.9). We confined the frequency of IL-6 Gene polymorphism by using Tetra-amplification refractory mutation system-polymerase chain reaction technique (TARMS PCR). Also, we determined the association of IL-6 Gene polymorphism in position (-174 G/C) with COVID 19 for Iraqi community. Data showed significant difference in genotypes frequency of IL-6 Gene polymorphism with COVID-19 patients and control.

The genotype of GG (homozygotes) show high frequency in COVID 19 patients was ratio 63.64%, comparison with control was 47.50% and showed related with etiological fraction risk of SARS-CoV-2 ratio was 30.7%, and OR (CI 95%) was 1.63(1.23 to 3.03). The genotypes of GC (Heterozygotes) show high frequency in control ratio was 50%, while in COVID-19 patients show low frequency was 32.72%, also GC genotypes showed relationship with preventive fraction risk of SARS-CoV-2 ratio was 25.7% and OR (CI 95%) was 0.49 (0.31 to 0.77). The CC genotype show non-significant between COVID-19 patients and control and ratio was 3.64% and 2.50% respectively, and was OR (CI 95%) 1.47 (0.38 TO 5.63).

The G allele show high in frequency of COVID-19 patients comparison with control ratio was 80% and 72.50% respectively, and present with etiological fraction risk of SARS-CoV-2, while C allele show low frequency in COVID-19 patients comparison with control ratio was 27.50% and 20% respectively, and present related with preventive fraction for SARS-CoV-2. Our findings demonstrate that the IL-6 gene in position -174 G/C may represent of SARS-CoV-2 risk development of COVID-19 patient’s in Iraqi population