HLA-DR and IL-17A serum concentration in pre and post-operative periods among Laparoscopic versus Conventional surgery in Iraqi Patients.

Abstract

Background: During the past decade, a number of experimental and clinical studies have provided evidence that surgical and trauma injury markedly affects the immune system, including both the specific and the nonspecific immune responses .Aim: the present study was conducted to evaluate the effect of conventional and laparoscopic surgery on HLA-DR lymphocyte and IL-17. Methods: A total of 50 patients were included in the study who were admitted to hospital from the period between March to August 2018 for different types of surgery, and divided into open surgery group (25) patients and laparoscopic surgery group (25) patients. Two blood sample were taken from each patients one before anesthesia and the other sample (12-24) hr. later postoperatively for HLA-DR lymphocyte flowcytometry and serum concentration of IL-17 by ELISA technique. Results: The count of CD8 lymphocytes, in Conventional Surgery group post-operative was significantly less than that of Laparoscopic surgery group, Mean serum concentration of IL- 17 in Conventional Surgery group post-operative was92.89±8.04 versus Laparoscopic Surgery group; 45.02±1.43. Conclusion: The results in the study showed that the HLA-DR expression was depressed in conventional surgery and significantly lower than that in patient with lap. Surgery intervention.