Identification of fungi associated with storage corn (zea mays) and estimate fumonisin B1 production by Fusarium spp.isolated from these cereals

Abstract

Zea mays one of the most important grains produced in Babylon province of Iraq incidences of contaminated seeds with toxigenic fungi were investigated in Babylon maize in 2016 from four Locations (centres (of accumelation corn seeds) namely medhateia, Babil, mussaieb and Locally markets, present study included Identificate the mycoflora of maize in these locations. Mycological analyses showed a predominance of Fusariumsverticilliodes (48.5%), followed by Aspergillus flavus (16.5%), F. proliferatum (6.5%), Penicillium species (5%), Mucor species (1.43%) in maize

Sixity strains of Fusarium verticillioides and F. proliferatum isolated from corn collected from fourtht stores in Babylon province, were evaluated for their ability to produce fumonisins B1 (FB1), in Rice culture. Fumonisin levels were determined by high-performance liquid chromatography.All tested strains of F.verticillioides and F. proliferatum produced fumonisins within a wide range of concentrations,233-1471ppb,176-823ppb,134-792 ppb and 236-1271ppb FB1. The highest mean concentrations of FB1 were 674,617,548and 340 ppb for location (localy markets,medhateia mussaieband and babil, , respectively) from the four locations of province were observed. Fumonisin mean levels produced byF. Verticillioides and F. proliferatum strains isolated from localy market zone) were significantly (P < 0.05) higher than the other three location.