The antagonistic effect of biosynthesized nanoparticles from aqueous extract of Agaricus bisporus on some pathogenic plant fungi

Abstract

The aims of this study was biosynthesized of AgNPs from aqueous extract of a fungus (Agaricus bisporus) and tests their efficacy against isolated fungi, samples were collected from various rotting fruits. Five types of fungi have been isolated, the types of fungi isolated are: Aspergillus niger , Aspergillu flavus, pancillium, mucori. All fungal isolated were identified by morphological and microscopic investigations. AFM was used for the characterization AgNPs size, the most average diameter was 33.40 nm. SEM was used for the characterization AgNPs size, the most average diameter was 50 nm. The effect of varied concentrations (1=30%AgNO₃-50%AE, 2=40%AgNo₃-50%AE, 3=50%AgNO₃-50%AE) of AgNPs , results demonstrated that AgNPs have a significant antifungal activity. A. niger showed that diameter of the colony in control was 30 mm, AgNps in concentration (1) as inhibitor for A. niger species, the diameter of the colonies reduced to 20.5 mm, concentration (2)15mm and concentration (3) 10 mm. The diameter of penicillium was 34.5 mm in the control sample. AgNps as inhibitor for Penicillium species, the diameter of the colonies of this species was 16.5 mm when treated with AgNPs at a concentration of (1), concentration (2) 15mm and concentration (3) 11mm. A. flavus, the average diameter of the colony was (21,25) mm on control sample. While when studying the effect of AgNps on A.flavus, the colony diameter was 19 mm when using AgNps concentration (1), concentration(2) 13mm and concentration (3) 7.5mm_. Diameter of the colony in the Mucor  was 30 mm. as control While the effect of AgNps at concentration (1) the diameter of the mucor colony was 16.5 mm , concentration (2) 15mm.