Isolation and Identification of Some Types of Bacteria Isolated from Different Pathological Conditions and Study of their Resistance to Antibiotics

Abstract

Infectious illnesses acquired in hospitals, known as nosocomial infections, caused by bacteria that have developed resistance to several antibiotics are a major public health concern around the worldThis bacteria is a common contaminant in healthcare facilities, where it has emerged as a powerful human pathogen. The goal of this research is to examine the antibiotic resistance patterns of two different species of bacteria—K.pneumoniae and A.baumannii. Since 170 human clinical samples were gathered from a variety of locations, we may say that (blood, pneumonia, wounds, urine). Bacterial culture, microscopic, and biochemical examinations, as well as examination with the Vitec device, revealed that 35 isolates exhibited characteristics typical of K.pneumoniae and that 16 isolates belonged to the type A.baumannii; subsequent testing of these isolates for resistance and sensitivity to 10 antibiotics, via the diffusion around the discs method, revealed that 33 K.pneumoniae and 9 A.baumannii isolates were resistant to Cefazolin, while baumannii is resistant to it, and 11 K.pneumoniae and 8 A.baumannii isolates showed resistance to the anti-piperacillin, while 5 isolates of each type showed resistance to the anti-Imipenem, and 10 K.pneumoniae and 6 A.baumannii isolates were resistant to Gentamicin, and 9 K.pneumoniae and 8 A.baumannii isolates were resistant to Ciprofloxacin, and 16 There were 24 K. pneumoniae and 9 A. baumannii isolates that were resistant to trimethoprim, and 5 K. pneumoniae and 11 A. baumannii isolates that were resistant to levofloxacin antagonist Tigecycline.