Synthesis and Characterization of the new 2- [2-(5-Nitro Thiazolyl) Azo]-4,6-Dibromo phenol (NTADBrP) as an Analytical Reagent for Determination of Copper (II) with Anticancer Study
Abstract
The new azo reagent 2- [2— -(5-nitro thiazolyl) azo] -4,6-Di bromo phenol (NTADBrP) was prepared and examined by using, UV-Vis, infrared spectra, H-NMR and mass spectrum. A sensitive and selective spectrophotometric method is proposed for the rapid determination of copper (II) using (NTADBrP) as anew spectrophotometric reagent. The reaction between this reagent and copper (II) is instantaneous at 589 nm (λmax) and pH=9 to form red complex having a mole ratio 1 : 2 (metal : Reagent) and the absorbance remains stable for over 24 hr. Beer's law is obeyed in the range of (1-40) μg.ml-1 with a detection limit of 0.3419μg.ml-1 The precision and accuracy were obtained to be R.S.D%=0.57%,Re%=97.02% and %Erel=2.98 %. By utilizing appropriate masking agents, the effect of ions (Co+2, Ni+2, Cr+3, Pb+2, Hg+2, Cd+2, Ag+1) that interfere with the reagent (NTADBrP) was evaluated, as well as its impact on the proliferation of laryngeal cancer cell lines and normal cells.
