Study the clinical and immunological parameters of Rabbits infected by Salmonella Typhimurium

Abstract

This study included the collection of (100) stool samples from patients ranging in age from 4 months to 40 years suffering from acute and bloody diarrhea, from different hospitals in Iraq for the period 10/2/2021 to 4/5/2022, and these samples were cultured on many media. Facultative, differential and enrichment cultures for the purpose of isolating salmonella bacteria, where (15) isolates of salmonella were obtained after it was diagnosed with the VITEK-2 device and its return was proven (5) isolates of non-typhoidal salmonella (Typhimurium). (40) local rabbits of different ages were used and divided into two groups, where the first group (20) rabbits to estimate the infected dose, the second group (10) rabbits were divided equally and used in experimental infection, as of the control group, and the infected group, where the infected dose was estimated to be equal to (5x107 ) and injected intraperitoneally For rabbits, where the experimental animals after the injection of the infected dose showed many clinical signs, including lethargy, diarrhea loss of appetite for food and weight loss, in addition to that,. As for the cellular and humoral immunological parameters, when conducting the DTH test, an increase in the thickness of the skin was observed 48 hours after the injection, while the phagocytosis index showed a significant increase in ingestion of yeast, while the IgG test showed an increase in the measurement of antibodies, which reached a height in the second, third and fourth week of experiments.