Comparative study of antibiotics resistance pattern in P. aeruginosa infection among patient from burn, wound and urine isolates in Iraqi hospitals

Abstract

The Pseudomonas aeruginosa considered as an opportunistic bacterium and one of the pathogen causing nosocomial infections affecting hospitalized patients. This study was performed on 90 isolates from burn, wound swabs and urine samples, 30 isolates from each source. The bacteria were isolated and Identification by used blood agar MacConkey agar, Nutrient agar, HiFluore Pseudomonas Agar Base and Cetrimide agar. Traditional biochemical tests were done. The VITEK-2 System for Identification of P. aeruginosa was used to prove a final identification. The susceptibility of the 90 isolates was tested by using a vitek2 compact system (kit VITEK 2 AST-GN, No. 222 cards. The biofilm ability of formation to act as a factor of virulence along with (MTP) method were used. The results indicated that, most isolates were capable to form biofilm. Higher P. aeruginosa percentage rate of isolates was from burin swabs (69.8%), all isolates were resisted strain, fallow by urine culture (60%) and wound swabs (49.2%). Most isolates of P. aeruginosa showed high prevalence of the antibiotic-resistance to wide range of antibiotics that has been used for treatment, especially, in burn followed by wound and urine. Furthermore, bacteria isolate in case of wound and urine infection from inpatient (hospitalized patients) were found to be highly resistance to almost all antibiotic used in this study while compared with the isolated from outpatient and there were no sensitive strain isolates from hospital. This study highlighted that should found control programs for mitigation P. aeruginosa circulation among patients admitted into hospitals, especially in burn centers.