Molecular Detection of Entamoeba Species of the Patients with Bloody Diarrhea in Al-Diwaniyah Province

Abstract

Amoebic dysentery is a human infection caused by Entamoeba histolytica pathogenic parasite. The present study utilized golden standard molecular detection technique a nested multiplex Polymerase Chain Reaction PCR on 112 stoolsamples collected from patients with bloody diarrhea in Al-Diwaniyah Province hospitals and private laboratories after microscopic diagnostic examination. Nested Multiplex PCR showed only 73 (65.2%) positive samples to E.histolytica , 61 (83.6%) of them are mono-infection and 12 (16.4%) are mixed infection with E.dispar and E.moshkovskii 9 (12.3%) and 3 (4.1%) respectively , while the data of Microscopy showed 66 samples are positive to EntamoebaSpecies leading to higher prevalence ofE.histolytica in Al-Diwaniyah Province when the methods of the molecular detection used instead of routine traditional microscopic examination , permitting to distinguish amongmorphologically identical human Entamoeba Species.