Evaluation the Association of Different Genotypes Variant of Transforming Growth Factor Beta 1 (TGIFβ1) and Fork Head Box Protein 3 (FOXP3) with Unexplained Females Infertility

Abstract

Background: infertility is failure to achieve a pregnancy in a 12-month period for patients under 35 years of age and failure to conceive in a 6-month period for the over 35 years.

Aim: The present study was conducted to investigate the association between multiple polymorphisms in TGF-β1 and FOXP3 by Investigate the role of polymorphisms of rs1800471 (G74C) of TGF-β1 gene, (rs2232368-G/A) of FOXP3 gene polymorphisms with the susceptibility and risk for unexplained female infertility and identify the power of Combined genotypes as a risk factor for infertility. Methods: A total of 50 female patients with unexplained infertility were included in the study who were admitted to hospital from the period November 2021 to the February 2022, and other groups consist of 50 apparently healthy individuals. A five ml of blood samples from each patients and control groups were collected by vein puncture using disposable syringes under aseptic technique, the five ml of each samples were transferred into with EDTA tube and immediately frozen at -20 ⸰Cuntil further use to avoid repeated thawing and freezing of sample for detection of TGFB1 and FOXP3 gene polymorphism by( Tetra ARMS-PCR) technique.Results: The results of the association between TGFB1gene polymorphism and risk of infertility revealed that the heterozygous genotype GC was considered as a risk factor for infertility according to odds ratio that equal to 4.329 (95% confidence interval of 0.84 -22.06) and an etiologic fraction of 0.769 . On the other hand, the homozygous genotype CC was a risk factor for infertility with an odds ratio of 6.184 (95% confidence interval of 0.69 – 55.21) and an etiologic fraction of 0. 838.While the association between TGFB1 allele polymorphism and risk of infertility observed there is a significant association between C allele and risk of infertility with p value equal to (P = 0.002). Therefore, C allele was a risk factor for infertility, on the other hand the association between FOXP3 gene polymorphism and risk of infertility is showed the heterozygous genotype GA was a risk factor for infertility according to odd ratio of 2.504 (95% confidence interval of 0.97 -6.46) and an etiologic fraction of 0.607, also the homozygous genotype AA was a risk factor for infertility with significant p value (P = 0.017). While the association between FOXP3allele polymorphism and risk of infertility is showed there is a significant association between the A allele and risk of infertility with p value (P = 0.001). Conclusion: A significant higher risk of developing infertility was observed in subjects with genotypes containing 3 or more risk alleles than those whose genotypes containing 0-1 risk alleles.