Pomegranate Juice Repairs Sperm Abnormities in Experimental Animals

Abstract

Pomegranate fruit (Punica granatum) has been consumed as a natural drug, especially in the Middle East, because of its health benefits. Pomegranate fruit has marked antioxidant activity and high levels of polyphenols compared to other fruits. Pomegranate juice (PJ) prevents the damage during spermatogenesis. The present study investigates pomegranate juice positive affection on reproductive system, oxidation and antioxidant activity. The study has been conducted on adult male mice at the department of physiology, College of Veterinary Medicine, Al-Qadisiya University during the period extended from October to April 2021. Sixty mature male mice (aged 90 days and weighted (25±10 g) were randomly assigned to 6 equal groups (10 each), control (C) males were drenched with drinking water daily for 60 days, Methotrexate treated males were drenched with suspension (mg/ kg b.w) daily for 35 and 60 days. Pomegranate juice treated males were drenched with suspension (50 mg/ kg b.w) daily for 20, 35 and 60 days. Methotrexate + Pomegranate juice (50 mg/ kg b.w) treated males were drenched with suspension daily for 35 and 60 days. Methotrexate + Pomegranate juice (100 mg/ kg b.w) treated males were drenched with suspension daily for 20, 35 and 60 days. Methotrexate + Pomegranate juice (200 mg/ kg b.w) treated males were drenched with suspension daily for 35 and 60 days. The present study showed a significant increase in serum testosterone concentrations after 20, 35, and 60 days compared with control. Epididymis samples obtained from Pomegranate juice treated group increases significant whither sperm status at 20-, 35-, and 60-day periods compared with control. In comparison between periods, a significant difference between periods and control group was recorded whereas at 20, 35, and 60 day periods compared with control treated group a significant increase in sperm status as the treatment period proceeded at the days 20th, 35th, and 6. In conclusion the Pomegranate juice antioxidant activity is of pharmacological value not only as an antioxidant but also as an inducer of endogenous enzymatic and non-enzymatic antioxidants by improving reproductive system expressed as levels of testosterone hormone and sperm status activity.