Isolation and Identification of Some Types of Pathogenic Bacteria from the Prepuce (Foreskin) of Circumcised Children in Samarra City/Iraq

Abstract

From July 15th through August 15th, 2022, researchers from Samarra University's College of Education's Biology Department in Samarra City, Salah Al-Din Governorate, Iraq, will be conducting their studies. The aims of this research are to identify the bacteria responsible for the mucosal injury in the prepuce and to develop a method for isolating and identifying them. Fifty bacterial samples are taken using a sterile swab from prepuce for circumcised male children's penises at a few private clinics in the city of Samarra, representing children aged one month to five years. Only 5 out of 50 samples did not provide any bacterial growth, for a 90% success rate. Diagnosis and biochemical testing for microorganisms in circumcised children's bark wounds reveal that Staphylococcus epidermis is the most prevalent bacterial isolate at 43.2%, followed by Staphylococcus aureus at 31.5%, Pseudomonas aeruginosa at 14.3%, and Escherichia coli at 11.5%. Circumcised children's bark is used to make tissue sections, and although the age of the kid has no influence on the composition of the tissue, the presence of certain bacteria does. These bacteria include S. epidermis, P. aeruginosa, S. aureus, and E. coli. Degeneration of the stratified squamous epithelium layer in the inner portion of the prepuce, aggregation of inflammatory cells inside the dermis, congestion, bleeding, and epithelial layers were all seen upon histopathological analysis. Some sections showed necrosis hyperplasia of stratified squamous epithelium, with aggregation of necrosing ells surrounding look and infiltration of inflammatory cells between the squamous epithelial tissues. According to the findings, bacteria play a crucial role in initiating tissue lesions. Bacteria such as S. epidermidis, P. aeruginosa, and S. aureus are among the leading causes of infectious balanoposthitis. Balanoposthitis most often manifests between the ages of 1 month and 5 years old in children.