Effect of Gold Nanoparticles on The Histology of Accessory Glands of Male Albino Rats Rattus Norvegicus

Abstract

Background: Gold nanoparticles [AuNPs] were highly used recently in many therapeutic approaches and medical applications, a few is known about the effects of those nanoparticles on prostate and seminal vesicles histology in animal model. Materials and methods: Thirty-five healthy male adult albino rat Rattus norvegicus aged 10–12-week-old with 200-230 body weight were used in the current study. Rats were randomly grouped into five groups: first group given distilled water, second group given 40µg/kg body weight of gold nanoparticles, third group given 80 µg/kg body weight of gold nanoparticles for 30 day; four group give 40µg/kg body weight of gold nanoparticles,five group give 80µg/kg body weight of gold nanoparticles for 60 days for all the administration done peritoneal injection. After the 24 of administration animals were sacrificed and their prostate and seminal vesicles were excluded and fixed in formalin then processed for light microscope examination. Results: Histological investigation of prostate,seminal vesicle of rats from treated group with 40 µg\kg,80µg/kg of body weight for 30 days revealed the presence of The section of the prostate shows an increased in alveoli folding,increased connective tissue(stromal) between the secretory alveoli and atypical hyperplasia,but the seminal vesicle where epithelial cell shrinkage with epithelial necrosis and cytoplasm vacuolation with atypia degeneration,as for injections for 60 day it leads to the expanding of secretory alveoli in prostate and their thin walls, accompanied by cell degeneration, and tissue sections of seminal showed shrinkage of the epithelial cells with atypical degeneration hyperplasia and degeneration of the sub-epithelial stromal. Conclusion: Our present study indicated that a dose of 80µg\kg of body weight of gold nanoparticles could cause a deleterious toxic effects on the prostate,seminal vesicle structure more prevalently than the dose of 40 µg\kg of body weight,referring that low doses could cause less toxic effects and dose related toxicity.