Inhibition of Invasion of Breast Cancer Cells by Glutaminase Extracted from Acinetobacter Baumannii

Abstract

Breast cancer is the second leading cause of death for women in Iraq after cardiovascular disease. Many studies have been conducted on the activities of plant and bacterial secondary metabolites and their role in inhibiting proliferation of cancer cells. However, there is no study has dealt with inhibiting the invasion of cancer cells. The invasion potential of cancer cells is the most dangerous criteria of cancer, as the ability of cancer cells to migrate from the place where the cancer originated to the rest of the body hinders the ability to recover from cancer and hinders the application of therapeutic and excisional techniques, which leads to death. Therefore, the current study aims to inhibit the migration of breast cancer cells and restrict their movement to facilitate their eradication or treatment using the enzyme glutaminase extracted from Acinetobacter sp. Ninety five samples of bacteria were collected and diagnosed using phenotypic and biochemical tests in addition to the diagnosis by (Vitec kit). The enzyme glutaminase was extracted, purified, and characterized from the diagnosed bacteria, and the enzyme’s effect on inhibiting the migration of breast cancer cells (MCF-7) and (MDA-231) was examined using a scratch assay, in addition to its effect on tumor cell proliferation using MTT assay. The results demonstrated the ability of the enzyme glutaminase to inhibit the migration and proliferation of breast cancer cells. It can be concluded that glutaminase has an antitumor effect in two different ways, the first is by inhibiting the migration of cancer cells and the second is by inhibiting the proliferation of cancer cells, making it a potential target that can be used as an antitumor therapy.