Effect of Nano Scale Titanium Dioxide in Quail Testes Tissue and Molecular Diagnosis in a RAPD_PCR Method

Abstract

This study was conducted at the Central Laboratory/Faculty of Agriculture and Forestry/University of Mosul with the aim of detecting the genetic effects of nano titanium dioxide TIO2NPs in the quail's testes using the RAPD-PCR index, The birds were treated from 10 to 50 days old with this substance, where the dosage was from one day to the next and DNA was extracted from the adult birds' testicles after sacrifice, where the samples were subjected to the polymerization chain reaction. (PCR) The results of RAPD-PCR interactions showed a clear difference in the DNA testicular sample of the quail bird treated with nano titanium in the concentration of 35PPM compared with the control group, If this substance has the ability to indiscriminate genetic changes that occurred during the duration of the dosage and a mutation, deformation or alteration in the genetic composition of DNA occurs in a given region, this is an indicator of gene alteration shown by random designed RAPD-PCR and randomly associated in certain places. The results of the control testicle and the treatment testicle of nano titanium have been associated with the same area but with a lower or more sequence in terms of the number of nitrogens bases according to the marker division sequence ranging from 250Pb to 10000pb, suggesting that the DNA had mutations because of nano titanium and there was a clear difference between them.