Immunohistochemistry Assay of Sodium Nitrate in White Mice Protected with Alpha Lipoic Acid

Abstract

Colorants, preservatives, stabilizers, antioxidants, thickeners, emulsifiers, acidity regulator agents, flavor enhancers, and anti-caking agents are all examples of food additives. Sodium nitrate is a white crystalline powder that is slightly hygroscopic. It used as a preservative, color fixative, and antimicrobial in the food industry, primarily in cheese, meats, and fish. The aim of this research was to study the immunotoxic effects of NaNO3 in addition to the antioxidant effects of α-lipoic acid in activating immunity and decreasing the toxic effect of sodium nitrate. Eighty adult male and female White mice, aged eight weeks, were separated into 4 groups:

The first group received 1/10 LD50 (302.2 mg per kg b.w.) sodium nitrate orally by gavage daily for 8 weeks. The second group received 30 mg per kg B.W of α-lipoic acid orally by gavage daily for 8 weeks. The third group received 1/10 LD50 (302.2 mg per kg b.w.) sodium nitrate and 30 mg/ kg b.w. α -lipoic acid orally by gavage daily for 8 weeks. The fourth group was the negative control group. The result exhibited that revealed significant increasing (p<0.05) level of CD4+T cell in animals treated with ALA (G2) compared to control(G4), (G1) and (G3) group.