Let-7a Induces to Apoptosis of Breast Tumor Cells by Up-Regulation of P53

Abstract

Let-7a is one of microRNA types which consider as suppressor of tumor in breast cancer, the low levels of let-7a in breast tumor that cause metastasis of tumor while the high expression that leads to reduce the tumor of breast cancer. In this study, we increased the expression of let-7a in MCF-7 breast tumor cell line by transfection this gene which cloned with topo-vector and detected the apoptosis by qRT-PCR for p53. We founded the apoptosis was increased in breast cancer cell line that treated with let-7a compared with the control. These data reinforce the suggestions which consider that let-7a as gene therapy in breast cancer.