Genetic Diagnosis of Candida Albicans from Patients with Renal Failure and the Ability to Produce Gliotoxin Mycotoxin

Abstract

The current study, which is carried out on patients with renal failure in Al-Najaf province, 200 oral samples were collected from the dialysis halls in males and females. In both genders, 75 positive samples were found,females have been shown to have a higher rate of Candida infection than males. Females have 48 positive samples out of 100, while males have 27 positive samples out of 100, the percentage of females in positive samples is 64 %, whereas the rate of males is 36%. a positive diagnosis of samples is made using molecular identification of C.albicans by PCR. The results of HPLC show ability of C.albicans to produce secondary metabolites represented by a similar structure of gliotoxin (GT), 16.33 µg/ml concentration is obtained as the highest concentration in the sample15 grown on Minimum Essential Medium (MEM) and low concentration in a sample 1 cultured on Sabouraud Dextrose Brot (SDB) is 1.568 µg/ml. The secondary metabolite of the extract was examined by FTIR, and it was found that there is a significant homogeneity in the active groups in GT.