Biosynthesis Silver Nanoparticles and use it as Antibacterial in Vitro

Abstract

The study included isolating Klebsiella pneumoniae from Al-Rifai General Hospital, Microbiology Department, and diagnosing it with biochemical tests. Then the bacterial supernatant was prepared from it in the laboratory and mixed with silver nitrate solution and kept in the incubator for 24 hours at a temperature of 37 Co and in a dark atmosphere in order to prepare (5 Mm) of silver nanoparticles solution. Then, the variation of color in the solution was observed as of yellow to brown, evidence of the establishment of silver nanoparticles, and then a UV-visible spectroscopy was performed, the result showed the peak with a wavelength of 252 nm. And make TEM test which are shows pseudo- spherical shape (5mM) and the size of nanoparticle ranges between 20 - 100 nm. Then was a sensitivity test against bacteria E. coli isolated from the hospital. the sensitivity test makes by agar well diffusion method, the result appears the effectiveness of the silver nanoparticles as an antibacterial against bacteria E. coli and measuring of a zone of inhibition ranging from 25 ml to 18 ml