The Relation of Oxidative Stress and Antioxidant Biomarkers in Serum Non-Alcohol Fatty Liver Disease adult and Obese Kids

Abstract

Background and objective: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of abnormal liver function and is characterized by hepatic steatosis in individuals with little or no alcohol consumption. The purpose of the study was to find the relationship between enzymatic and non-enzymatic antioxidants, lipid damage (MDA) and some biochemical parameters in 60 adult and 20 kids -patients with NAFLD.

Methods: This study included 160 patients and healthy control group, aged range from 5 to 75 years while BMI range from 15.9 to 50.9 Kg/m2, enzymatic antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)); non-enzymatic antioxidants (GSH, vit E and direct and total bilirubin); and lipid damage (malondialdehyde (MDA)), and biochemical markers ( liver enzyme ( ALT, AST & ALP), glucose, Albumin and lipid profile were determined in the serum samples.

Results: The activity of SOD, CAT, GPx, GSH and MDA and liver enzyme Alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP)), lipid profile increase excepted HDL were significantly elevated in adult and kids of NAFLD compared to controls (p<.001). Whereas decrease level of Vitamin E and ALB.

Conclusions: Our findings show the anti- oxidative stress-related parameters are markedly altered in the liver of NAFLD patients exhibiting high alterations in liver function tests, also dyslipidemia appear in total NAFLD adult and obese kids' patient groups.