Effect of Tropan Alkaloids in Cell Line in Vivo and Vitro in Mice Laboratory

Abstract

The purpose of this study was to see how the alkaloids tropan isolated from the leaves of Convolvulus arvensis affected the cancer cell line MCF 7 and the normal cell line MEF. The alkaloid Tropan has been reported to be found in plant leaves, however this study indicates that tropan is found in larger concentrations in Iraqi plants utilizing the TLC technique and solvent systems.

 On the cancer cell line MCF 7 and the normal cell line MEF, the cytotoxicity of Tropan alkaloid extract was examined. Tropan's toxic impact was determined by the rate of proliferation inhibition. At doses of 250 mg/ml, the Tropan extract inhibited MCF 7 cells at a higher proportion (36.67%). Tropan extract inhibits the pace of MEF cell line growth (11.3 % ).

This study, on the other hand, looked at the toxicity of Tropan in laboratory mice. The findings revealed that alkaloids extracts have a rather acute lethal impact in mice, with an LD50 of 79.52 mg/kg.

After (I.P) administration of alkaloids extract at doses of (0.2, 0.4, 0.8 mg/kg) for 30 days, the therapeutic impact of both extracts was investigated in tumor-bearing mice. The results demonstrated a (35.12 %) reduction in tumor volume in the animals treated with alkaloids extract, particularly at a dose of 0.8 mg/kg.