Evaluating Effectiveness of Pseudomonas Fluorescens and Serratia Marcescens against Galleria Mellonella

Abstract

During this study, some species of bacteria were isolated and diagnosed from Musca domestica on the outer surface and gut of flies, which were collected from different areas (vegetable and butcher shops) in Baghdad province. The most common isolates were found to be Pseudomonas fluorescens bacteria with a rate of 40%, followed by Staphylococcus lentus bacteria by 32%, and Serratia marcescens bacteria by 28%, thus confirming that Musca domestic is an important mechanical transporter that helps spread pathogens. The results showed that the best effective dilution in the first larvae of Galleria mellonella is 10-4 for Pseudomonas fluorescens bacteria with a mortality and smut rate of 15.62%, followed by a concentration of 10-6 with 12.5%, and then a concentration of 10-8 with a rate of 9.73% dead larva. As for the pupa, the 10-6 concentration gave the eclosion failure rate, followed by a 10-4 concentration, then a 10-8 concentration of 6.25% and 3.12%, respectively.

Serratia marcescens bacteria recorded the highest rate of larvae mortality and smut in concentration 10-6 at 12.5%, followed by a concentration of 10-8 with 9.73%, while the concentration of 10-4 gave the lowest rate of mortality and smut reached 3.12% dead larva, 10-4 concentration recorded the highest pupa emergence failure rate of 12.5%, followed by a concentration of 10-8, 10-6 with 6.25% and 3.12%.