Genetic Predisposition to Colitis and Immune Response in Mice Induced with Disease and Treated with Helminth Antigen

Abstract

The study aims to know the role of parasitic worm’s antigens in immunomodulation as its great importance in understanding the interactions between the host and the parasites, due to the immune modulation allows the survival of both the host and the parasite through the control of immune disorders and the sustainability of parasites. The worms have the ability to persist in the host and are also mainly responsible for the chronic infection, despite the strong immune response developed by the host-parasite. Here, the whole worm antigens was used treatment of one of autoimmune disease (colitis).The experiment included the use of 50 adult male albino mice , Mus musculus, with ages ranging between 8-10 weeks, average weights ranged between 18-32 g. The experiment includes inducing three groups (induced colitis mice, treated mice with worm’s antigens and control). The blood sample was collected from all individuals in all groups and divided into two samples one them used for measured of cytokines (IL-4,IL-10,IL-17) and the other was used for study of gene CXcl1 expression .The current study, in which (IL-4,IL-10) levels were measured, showed a clear and significant decrease in (IL-4,IL-10) levels in the ulcerative colitis induced group, While the results of the current study showed that the mean concentration of IL- 17 in the serum of the experimental group induced by ulcerative colitis was high compared to the control group and the other groups. The result of gene expression was discussion in this study and explain its relation with other criteria.