Spectrophotometric Determination of Amoxicillin in Pharmaceutical Preparations using In Aqueous Solution Alizarin Red S Reagent

Abstract

A simple, rapid and sensitive spectrophotometric method for the

Determination of amoxicillin was developed. The method is based on the

Proton transfer reaction with alizarin red sulphonate in aqueous neutral

Solution to form a violet product showing a maximum absorbance at

531nm with a molar absorptivity of 8091 L.mol-1.cm-1. Beer’s law is obeyed

Over the concentration range (1-20) μ g.ml-1 with the accuracy (average

Recovery) 100.51% and the precision (RSD) less than 2%. The method

Was successfully applied for the determination of amoxicillin in

Pharmaceutical formulation as injection and the results were in good

Agreement with the standard addition procedure.