Gene expression of Combination Effects of Phage-encoded Pseudolysin Protein and Antibiotics on MDR and XDR Bacterial Isolates

Abstract

Background and Objective: Bacterial resistance to antibiotics is a current heaӏthy probӏem that has claimed millions of people in recent years. Thus, the aim of present study was to evaluate the use cell wall lysis protein (endolysin) and determine if they could replace or enhancing the action of some of the current antibiotics used in treating some bacterial infections.

Materials and Methods: Different cӏinical specimens were coӏlected from patients to isolate and identify the bacterial isolates by using cultural,biochemical tests API systems and vitek-2 system followed by testing their resistance to antibiotics using antibiotic susceptibility test,then estimation the gene expression of efflux pump resistance genes when tested against antibiotic alone and with combination with prepared endolysin using real-time PCR.

Results: The resuӏts of antimicrobial activity of synthetic phage encoded endolysin (1 mg/ml) against MDR Escherichia coӏi,and klebsieӏla pneumoniae showed high efficient(p˂0.05) on the expression of efflux pump genes.On the other hand the activity of endolysin along with antibiotic showed a significant(p˂0.05) decrease in the expression of efflux pump genes. The results of gene expression for efflux pump resistance genes of Klebseilla pneumonia,and Escherichia coli gave high decrease in the expression when using endolysin, and when using endolysin with antibiotic compared with control isolates.

Conclusion:The synthetic endolysin revealed a significant effect as antimicrobial agent against MDR isolates and the effect of combination therapy of endolysin and antibiotic was synergistic effect and the expression of efflux pump genes were significantly decrease.