Isolation of Lactobacillus Spp. from First Trimester Pregnant and Determination their Antimicrobial Activity

Abstract

Objective: Lactobacilli species that are more adapted to the vaginal environment of women may colonize and defend against harmful bacteria in the vaginal area. Methodology: 90 isolate of Lactobacillus spp. were isolated anaerobically from bacterial vaginosis and non- bacterial vaginosis pregnant women in first trimester. Confirmation diagnosis by VITEK-2, the antibacterial activity of bacteriocin extracted from Lactobacillus spp. is estimated using susceptibility tests. Results: Two isolation species of Lactobacillus from pregnant women in first trimester are confirmed, 5 antibiotics are used against 10 isolates of Lactobacillus, 100% resist for Cefixime ,80% resist for Cefepime ,60% resist for Nitrofuranation ,60% resist for Amoxicillin/lavulanic, 30% resist for Amoxicillin. Antibacterial activity of bacteriocin are positive against tow pathogenic bacteria. Conclusion: Lactobacillus spp. is a probiotic when establishing and maintaining a healthy vaginal balance.