Antitumor Activity of Hesperidin Extracted from Citrus Limon

Abstract

Cancer is a group of diseases characterized by uncontrolled development and spread of abnormal cells and may be lead to death. Although the exact cause of most cancers is not well understood but it has recognized that the genetic aspect contributes to 5 to 10% of cancers. Other reasons Included poor diets, sure infections, lack of bodily activity, obesity, smokers, and pollution. Phytochemical analysis for plant extract were possessed flavonoids with different contents of 341.66±83.33 mg/ml for of Citrus limon. The reductive ability was more effective than trolox observrd the all concentrations in 0.64 mg/ml recorded the highest reductive ability in C. limon was 0.82±0.02 mg/ml. Also, the radical scavenging (DPPH) was significantly more effective in all concenteration than vitamin C as positive control, the concentrations 0.500 mg/ml represented the highest DPPH in Citrus limon was 97.53±1.43mg/ml. The flavonoid purified from Citrus limon by HPLC, as a possible induction of apoptosis that initiated by hesperidin treated AMJ13 cells was assessed by acridine orange- propidium iodide dual fluorescent staining. The results revealed that hesperidin induced cell death through apoptosis which detected by acridine orange-propidium iodide dual fluorescent staining assay, further confirmed its anticancer effects, suggesting potential applications of hesperidin and its derivatives as anticancer therapy.