Association between Genotypic and Phenotypic Formation of Biofilm in Pseudomonas aeruginosa Isolated from Diabetic Foot Patients

Abstract

The purpose of this research is to determine the relationship between genotypic and phenotypic characteristics associated with biofilm formation in Pseudomonas aeruginosa isolated from diabetic foot patients. Methods: The identification of Pseudomonas aeruginosa isolates was performed using morphology, microscopic examination, biochemical assays, and the vitek-2 system. In addition, the phenotypic detection of biofilm by CRA and TCP. Finally, biofilm genes (quorum-sensing) identification by PCR (Las I and Las R genes). Results: In accordance with the results obtained from 100 samples based on morphological and cultural characteristics as well as biochemical characteristics and the vitek-2 system only 47 (47%) isolates were identify as Pseudomonas aeruginosa. Biofilm formation found 41 (87.23%) isolates from Ps. aeruginosa able to produce biofilm by CRA and TCP are the number of isolates showing high biofilm formation was 33 (70.2%), moderate biofilm formation was 7 (14.9%) and non-biofilm formation was 7 (14.9%). Finally, the PCR data showed that LasI gene high prevalence 43/47 (91.4%), also LasR gene was positive in 33/47(70.2%) which encode for Biofilm formation in this study.