Molecular Evaluation of Acinetobacter Baumannii in A Sample of Iraqi Patients: Clinical and Environmental Study

Abstract

The current study included molecular evaluation of infection with Acinetobacter baumannii. During the period from (November 2020) to (June 2021), 200 different samples were collected from some hospitals in the city of Baghdad. (100) samples were from the hospital environment: 36% of them are isolated from patient beds, 14% from floors, 7% from tables, 5% from incubators, 18% from hospital equipment and 20% from standing water, In addition to (100) clinical samples from infected patients, 32% were isolated from sputum, 24% from urine, 16% from wounds, 10% from blood, 3% from burns and 6% fluids such as (spinal cord, pleural and peritoneum). Infections with Acinetobacter baumannii. Microscopic examination and culture diagnosis of bacteria were carried out, and the diagnosis was confirmed by complete biochemical tests, using the diagnostic kit API 20E, as well as using the VITEK2 Compact System, in addition to studying the genetic sequence of bacteria 16S rRNA to find out the diversity and genetic difference between bacterial isolates collected from environmental and clinical sources and knowing the ability of bacterial isolates to resist different antibiotics used in the current study.