Utilization of Spectrophotometric Technique for the Quantitative Evaluation of Doxycycline in its Pure form and its Therapeutic Doses

Abstract

A rapid and easy spectrophotometric system has been proposed to determine Doxycycline Hyclate (DOX) in pharmaceutical formulations. The oxidation strategy consists of putting Doxycycline with an excess quantity of N-Bromo succinimide (NBS). That happens in an acidic medium and then estimates the amount of unreacted NBS by methylene blue at λ580 nm. The quantity of NBS interacting with the colourant is equivalent to the amount of the drug with a better molar absorption of 2.44 × 10³ L. mol^-1 cm^-1. The bear law scale was 5-25 µg.ml^-1, and Sandell's sensitivity index of 0.210 g.cm^-2. And also, the average relative error of the concentration is -0.503 %, and the relative standard deviation is 0.337%. The method was successfully applied in its quantifying to the determination of drug in pharmaceutical formulations.