Comparing the Antifungal Effect of Hanse Dent Endomix with Ledermix and Metapaste on Candida Albicans: An In Vitro Study

Abstract

Aim of study: Candida albicans is one of the microorganisms that causes the failure of endodontic treatment, as it is stubborn to removal and resistant to many procedures in the canal system, so intracanal medicaments are used during the endodontic treatment to reduce its presence in the canal system, thus controlling one of the causes of endodontic treatment failure and relieving periapical lesions, so this study done to evaluate the effectiveness of three medicaments, which are Metapaste®, Ledermix® and Hanse Dent Endomix® against Candida albicans grown in pure cultures in Petri dishes within a special medium for its growth. Materials and Methods: Three Petri dishes A, B, and C were taken and divided into four parts: 1,2,3,4, each dish containing PDA culture medium. The medicaments were dissolved and impregnated on 6 mm diameter filter paper (Whatman paper) discs and then a suspension of Candida albicans was spread on the PDA medium in Petri dishes, Then the discs impregnated with medicaments were transferred to the dishes and placed over the fungal suspension. It was waited 24 hours of incubation at 37°C for the growth of Candida albicans, and then the results were taken. After clarifying the effectiveness of Hanse Dent Endomix® medicament and its effect on inhibiting the growth of Candida albicans, the medicament was extended to 6 diluted concentrations in glass tubes and impregnated these concentrations on 6 mm diameter Whatman discs. The effect of these concentrations on Candida albicans suspension in the PDA medium was studied to determine the minimum inhibitory concentration (MIC) of Hanse Dent Endomix®, within three groups of the experiment, namely, A, B, and C. Each experiment included two Petri dishes divided into eight sections. The first dish from each experiment was divided into 1,2,3,4. And the second dish was divided into 5,6,7. 8, The results were taken after 24 hours of incubation at 37°C. Results: Hanse Dent Endomix® medicament showed effectiveness in inhibiting the growth of Candida albicans, while Metapaste® and Ledermix® medicaments did not show any effectiveness in inhibiting the growth of Candida albicans fungi, the MIC  of Hanse Dent Endomix® after 24 hours is 9µg\ml. Conclusion: This study demonstrated the efficacy of Hanse Dent Endomix®, Metapaste® and Ledermix® in inhibiting the growth of Candida albicans, and determined the minimum inhibitory concentration of Hanse Dent Endomix® after 24 hours.