Effect of alcoholic extract edible mushroom Ganoderma lucidum on pathogenic bacteria isolated from children's' tonsils.

Abstract

Background: The research objective is to learn about inhibitory effect of the alcoholic extract for Ganoderma lucidum against five kinds of isolated bacteria from children's' tonsils which included Bacillus cereus, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Research Method: Alcoholic extract showed Inhibitory effect towards the growth of studied bacterial isolates and it was different by bacteria type and concentration by using the turbidity test method. Results: It reached on the concentration 200 mg/mL was the lowest value of turbidity recorded in B. cereus. Which gave a turbidity score (0.233) O.D. The inhibitory effect was in the turbidity way and at 630 nanometer wavelength and by using the alcoholic solvent (ethanol) in concentration 200 mg/ml best than the other concentrations. Effective anti-bacterial chemical compounds have also been detected in the alcoholic extract for G.lucidum using the GC-MS technique ,Special in chemical compounds separation and indicates the presence of the compound (E)-9- octadecenoic acid ethyl ester nearly 48.92%, It is an unsaturated fatty acid anti - bacterial inflammation. ‏Through molecular diagnosis, a new strain of studied bacteria obtained and registered B. cereus in the World Genetic Data Bank (NCBI) by Bacillus cereus As-A-M gene for 16S ribosomal RNA, partial sequence. Conclusion: Alcoholic extract also showed inhibitory activity of bacteria especially at concentration 200 mg/ml.